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Transfection Via Nucleofection

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Transfection Via Nucleofection

Author:   Pratheesh M D ,  Anand Laxmi N ,  Justin Davis K
Publisher:   LAP Lambert Academic Publishing
ISBN:  

9783846526293


Pages:   120
Publication Date:   13 October 2011
Format:   Paperback
Availability:   Not yet available   Availability explained
This item is yet to be released. You can pre-order this item and we will dispatch it to you upon it's release.
Online Price $82.70 RRP $97.28 Save $14.58 (15.00%) Quantity:  
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Overview

Transfection via Nucleofection is one of the most efficient non- viral method of introducing a foreign gene in to the host cell. Cell-type specific combinations of electrical current and solutions make this technology unique in its ability to transfer polyanionic macromolecules directly into the nucleus. Therefore, even cells with limited potential to divide are made accessible for efficient gene transfer. This monograph shows various optimization parameters and protocols for standardizing nucleofection protocol in goat fibroblast cells using bovine IFN- tagged with EGFP as the model gene construct. This technique can be utilized effectively for the production of animals with better production performances. In addition to the brief protocols of various biotechniques, this book offers a general review of the literatures dealing with the different ways of transfection, assay of transfection, with the hope that it will give compact information to the researchers and students involved in the reproductive biotechnology.

Full Product Details

Author:   Pratheesh M D ,  Anand Laxmi N ,  Justin Davis K
Publisher:   LAP Lambert Academic Publishing
Imprint:   LAP Lambert Academic Publishing
Country of Publication:   United States
Dimensions:   Width: 15.20cm , Height: 0.70cm , Length: 22.90cm
Weight:   0.186kg
ISBN:  

9783846526293


ISBN 10:   3846526290
Pages:   120
Publication Date:   13 October 2011
Audience:   General/trade ,  General
Format:   Paperback
Publisher's Status:   Active
Availability:   Not yet available   Availability explained
This item is yet to be released. You can pre-order this item and we will dispatch it to you upon it's release.

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